Describe reproducibly how to integrate measurments of sarcomere, YAP1 intensity, and cardiomyocyte area
How it was actually done before
I have a table where I somehow connected the measurments from cell area supplied by Katka and the sarcomere measurments clear
Outline
- Set up pipeline
- Run cells through pipeline
- Quality check
- Post process first part
- Tweak pipeline for the non-recognized cells
- Re-run cells through pipeline
- Quality check
- Post process second part
- Measure sarcomere length
- Process the treatmen_image-number_measurement-number
- Integrate with cell profiler measurements
- Set positivity treshold
- Split/label cells to positive/negative
- How to integrate multiple measurments of sarcomere length with a single value of cell area/yap1 intensity?
The most important question: How did I do it previously?